https://pages.htgaa.org/2026b/aleksandra-piotrowska/week-03-hw-cell-free/index.htmlOverview This week I worked on designing cell-free protein synthesis reactions for sfGFP, PETase and Reteplase. I started from Ginkgo reference formulations and used AI to help explain the biology, compare ingredients and check the volume calculations. My aim was to propose affordable reactions that could still produce functional protein. At this stage, I have submitted my sfGFP design and chosen a PETase design. The reactions have not been tested, and I do not yet have experimental results.Hugoen