https://pages.htgaa.org/2026b/alvaro-hernandez/index.htmlAbout me Alvaro Hernandez Zelada, student at National University of San Marcos, interested in immunomodulators, immunology, cancer, nanoparticlesand protein denovo design. Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/alvaro-hernandez/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/alvaro-hernandez/week-01-hw-project-ideas/index.htmlBiomanufacturing Homework 1 Folate-Targeted Lipid Nanoparticles Delivering CRISPR-Cas9 Against HPV16-Associated Cervical Cancer 1. What I Want to Make — and Why Product: Folate-receptor-targeted lipid nanoparticles (FA-LNPs) co-encapsulating Cas9 mRNA (modified with N1-methylpseudouridine) and two single-guide RNAs — sgRNA-E6 and sgRNA-E7 — directed against the HPV16 oncogenes responsible for cervical cancer. The final product is a sterile, injectable nanoparticle suspension of approximately 100 nm formulated in PBS with 10% sucrose as cryoprotectant.https://pages.htgaa.org/2026b/alvaro-hernandez/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/alvaro-hernandez/final-project/index.html