https://pages.htgaa.org/2026b/awais-ur-rehman/eek-03-hw-cell-free/index.htmlWeek 3 Homework – Designing Cell-Free Reaction Compositions Overview I designed cell-free protein synthesis (CFPS) reaction compositions for three proteins: sfGFP, PETase, and Reteplase using the coopetition.htgaa.org platform. Compositions Submitted 1. sfGFP Type: Reducing condition Energy source: PEP-based Comments: Simple PEP-based composition based on reference plate. 2. PETase Type: Reducing condition Energy source: PEP-based Comments: Similar to sfGFP but suitable for an enzyme with one disulfide bond. 3. Reteplase Type: Oxidizing (DSB) condition Special reagents: GSSG, GSH, and DsbC Comments: Oxidizing composition to support formation of 9 disulfide bonds. Community Reagent I suggested Trehalose as a community reagent. Reason: It acts as a protein stabilizer and can improve functional yield, especially for difficult proteins like Reteplase.Hugoen