https://pages.htgaa.org/2026b/awais-ur-rehman/index.htmlAbout me I’m a BS Biotechnology student at Virtual University of Pakistan, based in Pakistan. My main interests are synthetic biology, systems biology, bioinformatics, and biomanufacturing. I am currently building a strong foundation in molecular biology, genetics, and computational biology while exploring how biological systems can be designed and engineered for practical applications.Hugoenhttps://pages.htgaa.org/2026b/awais-ur-rehman/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/awais-ur-rehman/week-01-hw-project-ideas/index.htmlHomework 1: Biomanufacturing Idea using Xerophiles / Extremophiles (1) What I would like to biomanufacture and why Target product: Deinoxanthin (and related carotenoid antioxidants) produced by the xerotolerant polyextremophile Deinococcus radiodurans. Deinoxanthin is a unique carotenoid with strong antioxidant and radioprotective properties. It is more stable under oxidative stress and desiccation than many common carotenoids.https://pages.htgaa.org/2026b/awais-ur-rehman/week-02-hw-genetic-circuits/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/awais-ur-rehman/week-02-hw-genetic-circuits/index.htmlWeek 2 Homework – Genetic Circuit Design Part 0: Kernel Setup Repository: awais-deinoxanthin Folder: awais-deinoxanthin Part 1: Expression Target I designed a genetic construct to increase deinoxanthin production in Deinococcus radiodurans.https://pages.htgaa.org/2026b/awais-ur-rehman/eek-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/awais-ur-rehman/eek-03-hw-cell-free/index.htmlWeek 3 Homework – Designing Cell-Free Reaction Compositions Overview I designed cell-free protein synthesis (CFPS) reaction compositions for three proteins: sfGFP, PETase, and Reteplase using the coopetition.htgaa.org platform. Compositions Submitted 1. sfGFP Type: Reducing condition Energy source: PEP-based Comments: Simple PEP-based composition based on reference plate. 2. PETase Type: Reducing condition Energy source: PEP-based Comments: Similar to sfGFP but suitable for an enzyme with one disulfide bond. 3. Reteplase Type: Oxidizing (DSB) condition Special reagents: GSSG, GSH, and DsbC Comments: Oxidizing composition to support formation of 9 disulfide bonds. Community Reagent I suggested Trehalose as a community reagent. Reason: It acts as a protein stabilizer and can improve functional yield, especially for difficult proteins like Reteplase.https://pages.htgaa.org/2026b/awais-ur-rehman/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/awais-ur-rehman/final-project/index.html