https://pages.htgaa.org/2026b/caleb-seward/index.htmlAbout me I love growing things, on the weekends you can find me either gardening or in the forest looking for mushrooms to bring back home and clone. I am passionate about biotechnology and am looking forward to learning about automation and strengthening my knowledge of synthetic biology.Hugoenhttps://pages.htgaa.org/2026b/caleb-seward/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/caleb-seward/week-01-hw-project-ideas/index.htmlHTBAA Homework #1 — Recombinant human lactoferrin in Komagataella phaffii # 1. What I would like to biomanufacture and why I would like to biomanufacture recombinant human lactoferrin (hLF) using engineered yeast.https://pages.htgaa.org/2026b/caleb-seward/week-02-hw-principles-and-practices/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/caleb-seward/week-02-hw-principles-and-practices/index.htmlPart 1: Define your expression target ● Molecule: Human Lactoferrin, image obtained from UniProt (AlphaFold prediction) ● The sequence was obtained from uniprot (P02788 · TRFL_HUMAN), it is a 710 amino acid protein. Amino Acid sequence: MKLVFLVLLFLGALGLCLAGRRRSVQWCAVSQPEATKCFQWQRNMRKVRGPPVSCIKRDSPIQCIQAIAENRADAVTLDGGFIYEAGLAPYKLRPVAAEVYGTERQPRTHYYAVAVVKKGGSFQLNELQGLKSCHTGLRRTAGWNVPIGTLRPFLNWTGPPEPIEAAVARFFSASCVPGADKGQFPNLCRLCAGTGENKCAFSSQEPYFSYSGAFKCLRDGAGDVAFIRESTVFEDLSDEAERDEYELLCPDNTRKPVDKFKDCHLARVPSHAVVARSVNGKEDAIWNLLRQAQEKFGKDKSPKFQLFGSPSGQKDLLFKDSAIGFSRVPPRIDSGLYLGSGYFTAIQNLRKSEEEVAARRARVVWCAVGEQELRKCNQWSGLSEGSVTCSSASTTEDCIALVLKGEADAMSLDGGYVYTAGKCGLVPVLAENYKSQQSSDPDPNCVDRPVEGYLAVAVVRRSDTSLTWNSVKGKKSCHTAVDRTAGWNIPMGLLFNQTGSCKFDEYFSQSCAPGSDPRSNLCALCIGDEQGENKCVPNSNERYYGYTGAFRCLAENAGDVAFVKDVTVLQNTDGNNNEAWAKDLKLADFALLCLDGKRKPVTEARSCHLAMAPNHAVVSRMDKVERLKQVLLHQQAKFGRNGSDCPDKFCLFQSETKNLLFNDNTECLARLHGKTTYEKYLGPQYVAGITNLKKCSTSPLLEACEFLRK ● The living system is a yeast (Komagataella phaffii) ● The product needs to be excreted out of the cell to make purification easier. Part 2: Design your genetic partshttps://pages.htgaa.org/2026b/caleb-seward/week-03-hw-cfps/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/caleb-seward/week-03-hw-cfps/index.htmlA. Comparing compositions Potassium vs magnesium glutamate Magnesium glutamate is more sensitive. Potassium glutamate just sets the ionic background, approximating the cytoplasm the ribosome evolved in. The optimum is broad, so published values scatter from 130 mM in PANOx-SP to 362 mM in Olsen’s RFopt. Magnesium is a catalytic cofactor with a narrow window: free Mg2+ drives 30S/50S association, holds tRNA tertiary structure together, and is the catalytic metal for T7 polymerase and the synthetases. Too little and ribosomes don’t assemble, too much and elongation slows and fidelity drops. Across those same formulations it barely moves, 7 to 10 mM everywhere.https://pages.htgaa.org/2026b/caleb-seward/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/caleb-seward/final-project/index.html