https://pages.htgaa.org/2026b/camila-naranjo/week-03-hw-cell-free/index.htmlCFPS REACTION DESIGN FOR SFGFP, PETASE, RETEPLASE A. COMPARE CELL-FREE REACTION COMPOSITIONS 1. Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Potassium glutamate and magnesium glutamate contribute K⁺ and Mg²⁺ ions, respectively, which provide the ionic strength and the cofactors essential for activating the transcription and translation machinery, whilst the Glu⁻ anion is the predominant natural physiological anion in the cytoplasm of E. coli (1).Hugoen