<?xml version="1.0" encoding="utf-8" standalone="yes"?><rss version="2.0" xmlns:atom="http://www.w3.org/2005/Atom"><channel><title>Week 2 HW: DNA Design :: dakota-hamill</title><link>https://pages.htgaa.org/2026b/dakota-hamill/week-02-hw-dna-design/index.html</link><description>What molecule are you expressing? Lanmodulin, a protein.&#10;What is the sequence?&#10;ENA|ACS39628|ACS39628.1 Methylorubrum extorquens AM1 conserved hypothetical protein; putative exported protein ATGGCGTTCCGCCTCTCCTCTGCCGTTCTGCTCGCTGCGCTCGTCGCCGCTCCGGCCTAC GCCGCCCCGACGACGACCACGAAGGTCGATATCGCCGCCTTCGATCCGGACAAGGACGGC ACGATCGATCTGAAGGAGGCCCTGGCCGCCGGCTCCGCTGCCTTCGACAAGCTCGATCCG GATAAGGACGGCACGCTCGACGCTAAGGAGCTGAAGGGCCGCGTCAGCGAGGCCGACCTC AAGAAGCTCGACCCGGACAACGACGGCACCCTCGACAAGAAGGAATACCTTGCCGCCGTC GAGGCGCAGTTCAAGGCCGCTAACCCGGACAACGACGGCACCATCGACGCTAGGGAATTG GCCAGCCCGGCCGGTTCGGCCCTGGTCAATCTGATCCGCTAA&#10;https://www.uniprot.org/uniprotkb/C5B164/publications&#10;What chassis? E. Coli BL21 (DE3)&#10;Where does the product need to go? Preferably secreted outside of the cell.&#10;Part 2: Design your genetic parts Part Name Vector pUC-AmpR v4 backbone Promoter T7 Promoter RBS F1 RBS CDS LanM Tag BBa_K2539001 HisTag Terminator T7 Terminator I chose parts with the most simple names. I found Kernel hard to use, confusing, and with a poor UI.</description><generator>Hugo</generator><language>en</language><atom:link href="https://pages.htgaa.org/2026b/dakota-hamill/week-02-hw-dna-design/index.xml" rel="self" type="application/rss+xml"/></channel></rss>