https://pages.htgaa.org/2026b/daryoush-afshinjoo/week-03-hw-cell-free/index.htmlHugoenhttps://pages.htgaa.org/2026b/daryoush-afshinjoo/week-03-hw-cell-free/-_index/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/daryoush-afshinjoo/week-03-hw-cell-free/-_index/index.htmlWeek 3 HW: Cell-Free Protocols Assignment A: 1.Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Potassium glutamate and magnesium glutamate serve different purposes in a cell-free reaction. Potassium glutamate primarily helps reproduce the ionic environment of the E. coli cytoplasm, where potassium is the predominant intracellular cation. Magnesium glutamate supplies Mg²⁺, which is essential for processes involving ATP, nucleotides, ribosomes, and other components of transcription and translation. The concentrations therefore need to be balanced rather than simply maximized: changing them alters the chemical environment in which the cell-free machinery operates.