https://pages.htgaa.org/2026b/farrel-alfaza-marsetyo/week-03-hw-cell-free/index.htmlASSIGNMENT: CFPS REACTION DESIGN FOR SFGFP, PETASE, RETEPLASE A. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Ions can help or hinder protein expression in a system. In living cells ions could act as osmolyte which could disrupt osmotic balance between the cell and its environment, but as the cell free system is free of any semipermeable membrane this concern is irrelevant. However, ions could also act as other things, including cofactors to enzymes (especially metal ions), act as a barrier in vital processes that needs different concentration gradients (such as ATP producing systems), or disrupt ionic bonds between amino acid side chains in a protein. Potassium and Magnesium both are cations, and both have extensive biological functions. Potassium acts as the primary intercellular solute in many cells, while magnesium acts as cofactor for many enzymes and as a mimic for calcium. Therefore, I hypothesize that changes magnesium will have a greater effect towards the overall yield and thus is more sensitive. To test this, I plotted the Mg-Glu and K-Glu concentrations of each CFPS formulations in a graph and test their effect towards yield (with the help of claude), the resulting image is as follows:Hugoen