https://pages.htgaa.org/2026b/flavoris-belue/week-03-hw-cell-free/index.htmlCFPS REACTION DESIGN FOR sfGFP, PETASE, RETEPLASE A. COMPARE CELL-FREE REACTION COMPOSITIONS 1. Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? The concentrations of potassium glutamate and magnesium glutamate vary between CFPS formulations because the optimal concentrations depend on the other components in the reaction, especially the energy source being used. Potassium helps maintain an environment similar to the E. coli cytoplasm, while magnesium is important for transcription, translation, and ATP-dependent reactions.Hugoen