USER PROMPTS — OSK-mRNA Biomanufacturing Proposal Review
Conversation of 22 September 2026
Verbatim, in order. Attachments noted in [brackets].

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PROMPT 1 — 13:58
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[attached: OSK_mRNA_Biomanufacturing_Project_v2.docx]

This is a document proposing a project for how to biomanufacture almost anything.
you are a reviewer of said project, make sure that you optimize the following
prompt, check the validity of the background and methods mentionsd and

1. Critically assess all the assumptions made in this document.

2. for the references given in the reference section, make sure that the statement
matches the content of the reference. If you are not sure whether that's actually
the case, just based off the abstract and you don't have access to the full
document, feel free to ask for it.

3. ideally for all the assumptions that are made for calculations, base these
assumptions in the materials and methods part of a reference, instead of making
one up.

4. lastly I made some comments in curly parentheses. First discuss the comments I
made with me and then add the discussed proposal of the comments or like my actual
intent into the document.

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PROMPT 2 — 16:06
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[attached: main-4.pdf (Krusic et al. 2025, Mol Ther Nucleic Acids)]
[attached: s41467-020-15174-3.pdf (Sarkar et al. 2020, Nat Commun)]
[attached: elife-71624-v2.pdf (Gill et al. 2022, eLife)]

'2.1 sakar mention that its oskmln
'2.2 your recommendation is good
'2.6 see the attachment main-4
'3.2 qualitative assertion is enough
'3.3 i want it framed that the baseline automation is faster pipetting, but you
should also make a recommendation how to leverage automation better. you can
describe both proposed combinatorial screen
'3.4 your recommendation is good

I don't understand what you mean with one experiment

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PROMPT 3 — 16:11
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how would you run the experiment? I trust your judgement

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PROMPT 4 — 17:26
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does 4b answer the following questions:

Using your proposed living system and automation technology, estimate how long it
would take to manufacture that amount. Consider quantities such as production
rate, yield, volume, number of parallel experiments or reactors, automation
throughput, or whatever parameters are most appropriate for your system. You may
discover that the automation technology you would use to prototype your system is
very different from what you would use to manufacture at scale. If so, describe
both. Again, we are looking for a reasonable engineering estimate. Show your
assumptions and calculations.

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PROMPT 5 — 19:09
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extract the prompts into a txt file

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END
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