Week 2 HW: DNA Design

##Part 0: Set up in Kernel## Repository name: Gargi Project name: Deshmukh_mRNA biomanufacturing

##Part 1: Define your expression target##

I want to design a DNA construct to transcribe mRNA of Chandipura virus (CHPV) glycoprotein G. Chandipura virus is an emerging viral pathogen associated with encephalitic disease, particularly affecting children in parts of India. The viral glycoprotein G is an envelope protein involved in viral attachment and entry and is therefore an important antigenic component of the virus.

###Sequence:###

Chandipura virus isolate 1015405 glycoprotein (G) gene, complete cds GenBank: KY321565.1

ATGACTTCTTCAGTGACAATTAGTGTGATCCTTCTTATCTCCTTTATTACCCCATCATACTCATCTTTGAGTATAGCATTTCCAGAAAACACCAAATTAGATTGGAAGCCAGTCACAAAAAATACTAGATACTGCCCTATGGGTGGGGAATGGTTTCTAGAACCAGGGTTACAAGAAGAATCTTTCTTGAGCTCTACACCAATTGGTGCGACCCCCTCCAAGTCAGATGGATTTCTCTGTCATGCAGCCAAGTGGGTGACGACATGTGATTTCAGATGGTATGGACCCAAATATATTACACATTCAATTCATAATATCAAACCTACCCGATCAGATTGTGATACAGCGCTTGCATCGTACAAATCCGGGACATTGGTGAGCCCTGGTTTTCCCCCAGAGTCTTGTGGTTATGCTTCTGTGACTGACTCCGAGTTCTTGGTGATCATGATTACTCCTCATCACGTAGGTGTGGATGACTACAGAGGACATTGGGTAGATCCTCTTTTTGTTGGAGGAGAATGCGACCAGTCTTATTGTGATACTATCCACAACTCCTCAGTTTGGATTCCTGCTGATCAGACTAAGAAGAACATTTGCGGCCAGTCCTTTACCCCGCTGACTGTGACGGTTGCCTATGATAAAACCAAAGAAATCGCTGCAGGCGCACTAGTCTTTAAGAGCAAATATCACTCTCACATGGAAGGTGCTCGAACTTGCAGATTGAGTTATTGCGGTAGGAACGGAATTAAATTCCCCAATGGAGAGTGGGTCAGCCTGGATGTTAAAACTAAGATCCAAGAGAAACCTTTACTTCCCTTGTTTAAAGAGTGTCCTGCCGGGACAGAGGTGAGATCTACTCTTCAATCCGATGGGGCTCAAGTCTTGACCTCGGAGATTCAGAGGATTTTGGATTATTCCTTGTGTCAGAACACGTGGGATAAGGTAGAACGCAAAGAGCCGTTGTCTCCATTAGATCTCAGCTATTTGGCATCTAAATCTCCGGGGAAAGGTCTGGCATATACAGTGATAAATGGGACATTGTCATTTGCCCATACCAGATATGTGAGGATGTGGATTGATGGCCCGGTGTTGAAAGAAATGAAAGGCAAAAGGGAATCTCCTAGTGGGATCTCGAGTGATATTTGGACCCAATGGTTCAAATACGGGGATATGGAGATAGGTCCAAACGGCCTCTTAAAGACAGCAGGAGGGTACAAATTCCCCTGGCATCTGATCGGTATGGGAATTGTTGACAATGAACTACACGAGCTCAGTGAGGCAAACCCTTTAGACCATCCACAGCTACCTCATGCTCAGTCTATTGCCGACGATTCGGAGGAGATCTTCTTTGGAGACACTGGGGTTTCCAAGAATCCAGTAGAACTGGTTACAGGGTGGTTCACTAGCTGGAAAGAGAGCTTAGCTGCCGGTGTTGTTTTGATACTGGTAGTTGTCTTGATTTATGGTGTCCTCCGTTGTTTCCCGGTATTGTGTACTACCTGCAGAAAGCCCAAATGGAAGAAAGGGGTAGAGAGGTCCGATAGCTTTGAGATGCGGATTTTCAAGCCCAACAACATGAGAGCCAGAGTATGA

###Chassis:*** Cell-free system

###Where does the product need to go?### The product is purified CHPV-G mRNA obtained via in-vitriol transcription , however once it is translated inside a cell, it will make CHPV glycoprotein G.

CHPV-G is an envelope glycoprotein with protein-targeting and membrane-associated features. These features are encoded within the protein-coding region rather than being part of the mRNA’s UTRs.