https://pages.htgaa.org/2026b/gracious-isoah/index.htmlAbout me I have a background in microbiology, and I’m deeply interested in biotech entrepreneurship and venture building. I believe biotechnology can be a powerful tool for solving real problems across Africa, and I’m currently building the knowledge, skills, and experience I need to launch a biomanufacturing venture here on the continent.Hugoenhttps://pages.htgaa.org/2026b/gracious-isoah/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/gracious-isoah/week-01-hw-project-ideas/index.htmlhttps://pages.htgaa.org/2026b/gracious-isoah/week-02-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/gracious-isoah/week-02-dna-design/index.htmlHTBAA Homework #2: Recombinant Taq DNA Polymerase Expression Construct Part 0: Kernel setup I created the Kernel repository Gracious Osaibhue Isoah and the folder Parts folder for parts and Construct folder for construct in the HTBAA 2026 workspace. Figure 1. Kernel repository and project folder created for the Taq DNA polymerase expression-construct design.https://pages.htgaa.org/2026b/gracious-isoah/week-03-cfps/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/gracious-isoah/week-03-cfps/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Potassium glutamate and magnesium glutamate are water-soluble ionic salts added to the buffer of cell-free protein synthesis to mimic the cytoplasmic environment of the cell. They provide critical ions like Mg²⁺ and K⁺ at concentrations required for transcription, translation, ribosome function, and energy metabolism. Glutamate is generally a more physiologically suitable counter-anion than chloride for many bacterial CFPS systems. CFPS yields are known to be more sensitive to magnesium glutamate. It provides a more constrained variable, Mg²⁺. In a study by Cai et al., changes of about 1-2 mM in concentration can increase/decrease protein yield. Free Mg²⁺ is a core component of the translation machinery. It stabilizes rRNA and the functional structure of RNA. Other CFPS reagents, including ATP, GTP, and nucleic acids, bind to Mg²⁺ due to its chelating effect.https://pages.htgaa.org/2026b/gracious-isoah/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/gracious-isoah/final-project/index.html