Week 3 HW: Cell-Free Protocols
Background
In this assignment, you will design new reaction compositions for synthesizing three proteins (sfGFP, PETase, and Reteplase) through cell-free protein synthesis (CFPS). In lysate-based CFPS, the protein-synthesis machinery is extracted from cells and used outside of intact living cells.
This homework was designed based on an exciting ongoing project at Ginkgo Bioworks aimed at comparing the performance of human scientists and AI agents in designing cell-free protein synthesis reactions with the goal of optimising the cost of producing one gram of functional protein. Submitted reaction compositions will be part of a Coopetition.