https://pages.htgaa.org/2026b/helene-quispe-lafuente/week-01-hw-project-ideas/index.htmlPROPORSAL: Cell-free biomanufacturing applied to the production of precision enzymes for clinical diagnostics <What do you like biomanufacture and why?!– upload a nice image and put its filename inside the () above, then write up your homework here! –> The objective is to biomanufacture high-precision molecular biology enzymes, such as Taq DNA polymerases, reverse transcriptases, and restriction enzymes using living microbial systems to supply the clinical molecular diagnostics industry. This production is motivated by the high global demand for reliable, cost-effective diagnostic reagents required for detecting infectious diseases and genetic markers. Utilizing living cellular chassis allows for robust, high-yield protein expression through established fermentation pathways, making it much more economical for large-scale commercial distribution than other methods (Biotechnology Journal, 2024). What living system would you use? The manufacturing process relies on a living microbial chassis, specifically: genetically engineered strains of Escherichia coli. The production cycle begins by feeding the bacteria with low-cost carbon sources, nitrogen, and mineral salts inside a controlled bioreactor. Through standard recombinant protein expression, the bacterial cells grow rapidly and translate the target gene into the desired enzyme, accumulating it inside the cytoplasm or secreting it. How would you use automation automated micro-bioreactor systems. This technology is applied to the strain screening, media optimization, and fermentation monitoring phases. Automated liquid handlers allow researchers to test dozens of growth media recipes and induction temperatures simultaneously in parallel wells, optimizing cell growth How much would you need to make, and how long would it take? Ideally to estimate production scale, a standard industrial fermentation run utilizing a 10-liter bioreactor can achieve typical bacterial cell densities yielding roughly 5 grams of target purified enzyme per batch. Supplying a regional diagnostics network requiring 10,000 tests monthly (utilizing 1 microgram per test, totaling 10 milligrams). From inoculation to final downstream purification, a complete fermentation and recovery cycle takes approximately 1/2 or 1 month. What don´t you know yet? How can I scale up taking into account different variables to ensure optimal performance? I am also unaware of the potential consequences of over-expression of these enzymes in a living system.Hugoen