{
  "sample_id": "ilknur_icke_2026-10-06_petase_pep_dsbc2p5_v1",
  "sample_type": "experimental",
  "reagent_list": {
    "lysate": 5000,
    "template_petase": 2000,
    "base_buffer": 2000,
    "magnesium_glutamate": 300,
    "ammonium_glutamate": 200,
    "amino_acid_mix_17": 400,
    "hepes_koh": 550,
    "pep_mono": 6000,
    "folinic_acid": 50,
    "coa": 100,
    "nad": 75,
    "putrescine": 50,
    "spermidine": 125,
    "potassium_oxalate": 150,
    "dsbc_ecoli": 500,
    "atp": 250,
    "ctp": 175,
    "gtp": 175,
    "utp": 175,
    "nuclease_free_water": 1725
  },
  "metadata": {
    "ai_model": "Codex (OpenAI); exact model version not recorded",
    "protein_target": "PETase",
    "acknowledgements": "AI-assisted design based on the course PETase PEP reference (Cutinase_R2PC_016), selected from the AI-simulated practising senior scientist example. No experimental results are claimed.",
    "comments": "Baseline: PETase PEP reference Cutinase_R2PC_016. Reduce the 100 uM DsbC stock from 1,000 to 500 nL (5 to 2.5 uM final); water becomes 1725 nL. All other additions remain unchanged. Total 20,000 nL; all transfers use 25 nL increments.\n\nThis tests whether a smaller DsbC dose retains enough enzyme activity to justify using less helper. The published PETase control plate held DsbC at 1,000 nL in all 312 experimental wells; no exact candidate match was found there. Compare with the unchanged reference under matched conditions. Activity and cost per activity remain unknown; 50% less DsbC does not mean 50% lower total cost.\n\nAI client: Codex (OpenAI). Original user prompt: \"ok now create 3 agents, one undergraduate biology, one graduate level and one practising senior scientist and one very expert level wet lab scientist and have them create their versions of completed homework in independent mode\". The example was subsequently revised against the course inventory. The authoring and revision prompt record is retained in the homework documentation."
  }
}