{
  "sample_id": "ilknur_icke_2026-10-06_reteplase_pep_dsbc3p75_v1",
  "sample_type": "experimental",
  "reagent_list": {
    "lysate": 5000,
    "template_reteplase": 2000,
    "base_buffer": 2000,
    "magnesium_glutamate": 225,
    "ammonium_glutamate": 200,
    "amino_acid_mix_17": 400,
    "hepes_koh": 550,
    "pep_mono": 6000,
    "folinic_acid": 50,
    "coa": 100,
    "nad": 75,
    "putrescine": 50,
    "spermidine": 125,
    "oxidized_glutathione": 475,
    "reduced_glutathione": 200,
    "potassium_oxalate": 150,
    "dsbc_ecoli": 750,
    "atp": 250,
    "ctp": 175,
    "gtp": 175,
    "utp": 175,
    "nuclease_free_water": 875
  },
  "metadata": {
    "ai_model": "Codex (OpenAI); exact model version not recorded",
    "protein_target": "Reteplase",
    "acknowledgements": "AI-assisted design based on the course Reteplase PEP reference (Reteplase_R2PC_041), selected from the AI-simulated undergraduate biology example. No experimental results are claimed.",
    "comments": "Baseline: Reteplase PEP reference Reteplase_R2PC_041. Reduce the 100 uM DsbC stock from 1,000 to 750 nL (5 to 3.75 uM final); water becomes 875 nL. All other additions remain unchanged. Total 20,000 nL; all transfers use 25 nL increments.\n\nThis tests whether a smaller DsbC dose retains enough enzyme activity to justify using less helper. The published Reteplase control plate held DsbC at 1,000 nL in all 312 experimental wells; no exact candidate match was found there. Compare with the unchanged reference under matched conditions. Activity and cost per activity remain unknown; 25% less DsbC does not mean 25% lower total cost.\n\nAI client: Codex (OpenAI). Original user prompt: \"ok now create 3 agents, one undergraduate biology, one graduate level and one practising senior scientist and one very expert level wet lab scientist and have them create their versions of completed homework in independent mode\". The example was subsequently revised against the course inventory. The authoring and revision prompt record is retained in the homework documentation."
  }
}