https://pages.htgaa.org/2026b/jennifer-zayetsy-almeyda-tejada-almeyda/week-02-hw-dna-design/index.htmlHugoenhttps://pages.htgaa.org/2026b/jennifer-zayetsy-almeyda-tejada-almeyda/week-02-hw-dna-design/hw2_index/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/jennifer-zayetsy-almeyda-tejada-almeyda/week-02-hw-dna-design/hw2_index/index.htmlProgrammable CHO cell-state control for biomanufacturing Jennifer Zayetsy Almeyda Tejada My project asks whether synthetic gene regulation combined with AI-guided cell-state modeling can be used to program CHO cells to transition from a growth-oriented state to a production-oriented state during biomanufacturing. For this homework, I reduced the larger project to a smaller and experimentally interpretable proof-of-concept genetic circuit. The current construct uses constitutive expression of rtTA3G together with a TRE3G-controlled XBP1s reporter cassette. Doxycycline provides an external timing input, allowing the production-associated module to be activated at a selected point during culture.