https://pages.htgaa.org/2026b/jose-david-toledo-novoa/index.htmlHi, I’m David! 👋 Bioengineering Student · UTEC · Lima, Peru 🇵🇪 Welcome to my little corner of HTGAA, where I’ll be documenting my journey through synthetic biology, biomanufacturing, and everything in between. About me I’m Jose David Toledo Novoa, a fourth-year Bioengineering student at the University of Engineering and Technology (UTEC) in Lima, Peru.Hugoenhttps://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-01-hw-project-ideas/index.htmlExploring bacterial cellulose as a biomaterial for wound dressings, from microbial production to automation and scale-up.https://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-02-hw-dna-design/index.htmlHTBAA Homework #2: DNA Design Part 0: Set up in Kernel My project is stored in the David-Toledo-Bacterial-Cellulose repository, inside the toledo-bacterial-cellulose folder. Part 1: Define your expression target Expression target My project aims to produce bacterial cellulose for wound-dressing applications. Since cellulose itself is not a protein, I chose to express BcsA and BcsB, two core components of the bacterial cellulose synthase complex.https://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/jose-david-toledo-novoa/week-03-hw-cell-free/index.htmlWeek 3 — Cell-Free Reaction Design 3. Assignment: CFPS Reaction Design for sfGFP, PETase, and Reteplase The objective of this assignment was to design three cell-free protein synthesis (CFPS) reaction compositions, one for each target protein: sfGFP, PETase, and Reteplase. The main challenge was not simply to maximize protein production, but to reduce the cost of producing functional protein. For this reason, I compared different energy systems, considered the folding requirements of each protein, and identified reagents that could potentially be reduced without significantly affecting protein activity.https://pages.htgaa.org/2026b/jose-david-toledo-novoa/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/jose-david-toledo-novoa/final-project/index.html