Week 2 HW: DNA Design

PART I: DESIGN: DEFINITION OF EXPRESSION TARGET

What molecule are you expressing?

What is the sequence?

What host or chassis?

Where does the product need to go?

PART II: DESIGN: DEFINITION OF GENETIC PARTS

Layer 1: Transcription start

. Part Short Name:
. Sequence:
. Origin:
. Justification:

Layer 2: Translation start

. Part Short Name:
. Sequence:
. Origin:
. Justification:

Layer 3: Coding sequence

. Part Short Name:
. Sequence:
. Origin:
. Justification:

Layer 4: Targeting

. Part Short Name:
. Sequence:
. Origin:
. Justification:

Layer 5: Transcription stop

. Part Short Name:
. Sequence:
. Origin:
. Justification:

Layer 6: Vector context

. Part Short Name:
. Sequence:
. Origin:
. Justification:

PART III: DESIGN: CONSTRUCT ASSEMBLY

Kernel Schematic:

Link to Kernel Assembled Construct:

PART IV: DESIGN: OPTIMIZATION & QUALITY CHECK

Codon Optimization:

. BEFORE:
. AFTER:

Sequence Search:

Safety Screen Report:

PART V: FROM DESIGN TO BUILD

How would you build it?

What are your synthesis constrains?

Prototype vs. scale: