https://pages.htgaa.org/2026b/malinche-mejia-medina/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/malinche-mejia-medina/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/malinche-mejia-medina/week-01-hw-project-ideas/index.htmlAutomated Biomanufacturing of Tissue-Mimicking Phantoms for Electrosurgical Device Testing 1. What do you want to make and why? I want to develop a multilayer tissue-mimicking phantom for testing and optimizing electrosurgical devices.https://pages.htgaa.org/2026b/malinche-mejia-medina/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/malinche-mejia-medina/week-02-hw-dna-design/index.htmlDNA Design Part 0: Set up in Kernel The project workspace is established in the Kernel HTBAA 2026 workspace under the repository “HW 2 Malinche Mejia Gellan Tissue Phantom” and folder “Mejia-GellanPhantom”. In Kernel, a Part is an individual DNA element used for genetic design, such as a promoter, ribosome binding site (RBS), coding sequence (CDS), or terminator. A Construct is a complete DNA design assembled from multiple Parts in a specific order.https://pages.htgaa.org/2026b/malinche-mejia-medina/week-03-hw-cell-free-protocols/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/malinche-mejia-medina/week-03-hw-cell-free-protocols/index.htmlSection 3: Cell-Free Reaction Design Assignment A. Compare Cell-Free Reaction Compositions 1. Potassium Glutamate vs. Magnesium Glutamate Potassium Glutamate (K-Glu): Role: Supplies K⁺ (monovalent cation) and glutamate⁻ (organic anion). K⁺ is required for ribosome stability, codon-anticodon decoding, and peptide bond formation. Glutamate mimics the natural intracellular anion environment of E. coli, minimizing enzyme denaturation compared to chloride (Cl⁻). Concentration Range: Typically 60–180 mM (optimal around 100–130 mM). Sensitivity: Possesses a broad bell-shaped curve. Moderate variations (±10–20 mM) lead to minor, gradual changes in translation yield. Magnesium Glutamate (Mg-Glu):https://pages.htgaa.org/2026b/malinche-mejia-medina/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/malinche-mejia-medina/final-project/index.html