https://pages.htgaa.org/2026b/malinche-mejia-medina/week-03-hw-cell-free-protocols/index.htmlSection 3: Cell-Free Reaction Design Assignment A. Compare Cell-Free Reaction Compositions 1. Potassium Glutamate vs. Magnesium Glutamate Potassium Glutamate (K-Glu): Role: Supplies K⁺ (monovalent cation) and glutamate⁻ (organic anion). K⁺ is required for ribosome stability, codon-anticodon decoding, and peptide bond formation. Glutamate mimics the natural intracellular anion environment of E. coli, minimizing enzyme denaturation compared to chloride (Cl⁻). Concentration Range: Typically 60–180 mM (optimal around 100–130 mM). Sensitivity: Possesses a broad bell-shaped curve. Moderate variations (±10–20 mM) lead to minor, gradual changes in translation yield. Magnesium Glutamate (Mg-Glu):Hugoen