https://pages.htgaa.org/2026b/mariana-palazuelos-prado/index.htmlAbout me I’m a Costa Rican student pursuing a Bsc in Engineering in Industrial Bioprocesses at Universidad Nacional de Costa Rica. I have given myself a chance to develop skills in different areas within STEM to support my academic endeavours. I have certifications in Good Clinical Practices, Business Analytics and Big Data, AI and STEM applications, Automation and Quality Testing, Web Development and Project Management. My career has allowed me to use tools such as R Studio, Python and Polymath.Hugoenhttps://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-01-hw-project-ideas/index.html(1) What would you like to biomanufacture and why? I would like to produce second-generation biobutanol from de-oiled citrus waste sourced from CENADA (National Centre for Food Supply and Distribution). This biofuel will be produced via ABE (Acetone-Butanol-Ethanol) fermentation, supported by automated systems. This project aims to generate alternatives to fossil fuels given current geopolitical disruptions in oil distribution, while supporting Costa Rica’s goals under the National Decarbonization Plan.https://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-02-hw-asimov-integration/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-02-hw-asimov-integration/index.htmlPart 0: Set up in Kernel The construct and associated genetic parts are stored within the repo named palazuelos-biobutanol under HTBAA 2026 workspace URL: https://kernel.asimov.com/htgaa-2026/repositories/repository/c5bb3642-7d18-4fd7-a216-877eec535685 Part 1: Define your expression target ● What molecule are you expressing? A multi-subunit Resistance-Nodulation-Division (RND) family organic solvent efflux pump complex (specifically the TtgABC tripartite efflux system) alongside its transcriptional repressor TtgR. This complex extrudes monoterpenes (residual D-limonene <0.05%) and accumulating short-chain alcohols (biobutanol) directly out of the cell envelope.https://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mariana-palazuelos-prado/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Explain how a PEP+NTP reaction formulation is different from a Ribose+NMP reaction formulation. Choose two cell-free reaction compositions from the literature, compare and contrast the choice of reagents and the concentration of reagents. B. DESIGN ONE REACTION COMPOSITION FOR EACH OF THE THREE PROTEINS (40 minutes)https://pages.htgaa.org/2026b/mariana-palazuelos-prado/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mariana-palazuelos-prado/final-project/index.html