Week 3 HW: CFPS Design

HTBAA Week 3: Designing a Cell-Free Reaction Composition

Iterative experimental design

Matthew Woslum  -  September 22, 2026

Part A: Compare Cell-Free Reaction Compositions

Potassium Glutamate vs. Magnesium Glutamate:

Potassium maintains ionic strength and protein synthesis, while magnesium is essential for transcription and translation.

PEP+NTP vs. Ribose+NMP Formulation:

PEP directly regenerates ATP, while ribose and NMPs require cellular machinery to synthesize NTPs.

Literature Comparison:

*in progress*

Part B: Design Reaction Compositions

*in progress*