Week 2 HM: DNA Design
DNA Design - S100 Proteins
Part O - Set up Kernel
“(Hydrox)apatine(HAp) Hexoesqueletons from S100 Proteins”
“Part 1 - Define your expression target”
“I. What molecule are you expressing”?
“A helix-bucle-helix type of composed by two unions of calcium. However, I will try to express the Hyalin - HYAL_STRPU for this experiment.”
“Protein: Hyalin FUNCTION: Major constituent of the hyaline layer. The hyaline layer of echinoderm embryos is an extraembryonic matrix that functions as a substrate for cell adhesion through early development. SUBUNIT: Homooligomer in presence of calcium. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. PTM: Glycosylated. REFERENCE: KERNEL Repository”
“II. What is the sequence?”
“SSHNPGQSFTTGTTTTVVYTATDAFANVGQCAFTITVTATDTTPPVVTVSSSTITRQVEVGTPGVNVFFTEPTATDNSGQAILISRTNQPGDFFSVGQTIVTYTFQDPSGNPASGTVTINVIEVDTTPPVVTVPSTTITRQVEVGSSGVNVFYTEPTATDNSGQAILTSRTNQPGDFFPVGQTTVTYTFQDPSGNPASGTVTINVIEVDTTPPVVTVPSTTITRQVEVGSSGVNVFYTEPTATDNSGQAILTSRTNQPGDFFPVGQTTVTYTFSQDPSGNPASATVTINVIEVDTTPPVVTVPSTTITRQVEIGTPGVNVFYTEPTATDNSGIANLVSRTNQPGDFFPVGQTDVTYTFQDPSGNPSSGTVTINVVEVDTTSPVITVPSNTITREVELGTPGVNVFYTEPTATDNSGIANLVSRTNQPGDFFNVGQTVVTYTFQDPSGNPASGTVTINVVEVDTTPPQVFVTTGEVVRTAPFGSSGVNVFFTEPVAIDNSGTADLVSQTAQPGDFFPIGSTVVTYRYRDASGNEATGTFTVTVEEMVDTTPPTVTVSGGNIVRTVELGQSRLSVIYTEPTATDNSGEANLVSRTAQPGDLFPVGTTTVEYVYQDPAGNEGRGQFTVTVIAVDTTPPSVNCPNNVFQEVELGTSSAPVFFTEPTAFDISGQANLVTRTAAPGDSFPVGTTSVSYIFSDNSGNEAEPCTFTVTISAVDTTPPTVNCINNVAQTVQLGTGSTQVFFTEPTAFDISGQTSLVTRTSAPGDSFPVGTTSVTYIFTDASGNNAQPCTFNVVINAVDTTPPTVNCANNIAQTIELGSTSAVVNYAEPSATDISGTAFLVSRSSSPGDSFPIGSTTVTYIFSDQSGNEAPPCVFTVTIGTVDTMMPMFTSCPNNIVQSVELGVPGTVISWTTPTANDAAGIASIVSNLQPGSFFTVGESATVTYVATDNSGLTDNSCSFTVTVFAVDTTPPSVVCTNNVFQTVELGTNPVQVFYTEPTASDISGQANLVSRTNVPGDSFPVGTSTVTYVFADNSGNTADACSFTITVTAQDTTPPTVNCITNIVRTVELGTSSVQVFYNEPTASDLGQASLVSRTAQPGDNFPVGVNPVTYVFGDNSGNQADPCTFTITVNTQDTTPPTVTCISDITRVVELGTTSVGISYTEPTATDISGTATLDSRSHTPGQNFPVGSTVVSYFFSD” “REFERENCE: KERNEL Repository”
“III. What host or chassis?”
“Organism: Strongylocentrotus purpuratus (Purple sea urchin)”
“Where does the product needs to go?”
“Part 2. Design your genetic parts”
“I. Transcriptio start:”
“II. Translation start”
“III. Codign sequence:”
“IV. Targeting: (if need it):”
“V. Transcription stop:”
“VI. “Vector context:”