https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-01-hw-project-ideas/index.html1. What do you want to make? → An engineered trehalose-overproducing rhizobacterium, applied as a dry seed coating on rainfed wheat, that manufactures drought-priming signal continuously at the root instead of delivering it once as a chemical. 2. What living system could make it? → Azospirillum brasilense (or a Bacillus backup for shelf life) carrying a bifunctional yeast TPS–TPP fusion under an osmotically inducible promoter. In: seed, soil water, root exudate carbon. Out: a colonized, stress-primed root system.https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-02-hw-dna-design/index.htmlPart 0: Setup in Benchling Repository Name: Project/MIT HTBAA 2026 Project Name: Engineered trehalose-overproducing rhizobacterial seed inoculant for rainfed wheat Part 1: Expression Target What molecule am I expressing? A single bifunctional trehalose-6-phosphate synthase / trehalose-6-phosphate phosphatase (TPS–TPP) fusion enzyme of 892 amino acids: one polypeptide carrying two sequential catalytic activities. TPS (EC 2.4.1.15) condenses UDP-glucose and glucose-6-phosphate to trehalose-6-phosphate (T6P); TPP (EC 3.1.3.12) then dephosphorylates T6P to trehalose. Expressing this as one fusion rather than two genes is a deliberate design choice with three justifications, two of them drawn directly from the source literature:https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-03-hw-cell-free/index.htmlSection A A1. Why K-glutamate and Mg-glutamate concentrations differ — and which is more sensitive Potassium Glutamate (K-Glu, ~100–200 mM) — Bulk Counterion: Serves as the primary salt to set the ionic strength and osmolarity to mimic the cellular environment (E. coli cytoplasm) and stabilize ribosome/tRNA structures. It has low sensitivity because its optimal range is broad and forgiving (typically ±30–50 mM).https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/final-project/index.html