https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-02-hw-dna-design/index.htmlPart 0: Setup in Benchling Repository Name: Project/MIT HTBAA 2026 Project Name: Engineered trehalose-overproducing rhizobacterial seed inoculant for rainfed wheat Part 1: Expression Target What molecule am I expressing? A single bifunctional trehalose-6-phosphate synthase / trehalose-6-phosphate phosphatase (TPS–TPP) fusion enzyme of 892 amino acids: one polypeptide carrying two sequential catalytic activities. TPS (EC 2.4.1.15) condenses UDP-glucose and glucose-6-phosphate to trehalose-6-phosphate (T6P); TPP (EC 3.1.3.12) then dephosphorylates T6P to trehalose. Expressing this as one fusion rather than two genes is a deliberate design choice with three justifications, two of them drawn directly from the source literature:Hugoen