https://pages.htgaa.org/2026b/mehmet-erkan-ozseyhan/week-03-hw-cell-free/index.htmlSection A A1. Why K-glutamate and Mg-glutamate concentrations differ — and which is more sensitive Potassium Glutamate (K-Glu, ~100–200 mM) — Bulk Counterion: Serves as the primary salt to set the ionic strength and osmolarity to mimic the cellular environment (E. coli cytoplasm) and stabilize ribosome/tRNA structures. It has low sensitivity because its optimal range is broad and forgiving (typically ±30–50 mM).Hugoen