https://pages.htgaa.org/2026b/michellie-hernandez/index.htmlAbout me I am a medical doctor in the Dominican Republic. I ❤️ learning and consider myself a lifelong learner. I have a blog called MD Biomimicry integrating medicine, AI and Biomimicry where I edit AI generated content and add my own ideas and research suggestions. In 2020 I came up with the HOPE method; reverse engineering antibodies from cryo-EM and mass spectrometry data with de novo synthesis and published a more detailed paper in 2023 with a preprint in 2022. Since then I have a strong interest to transition into the field. I suffer from an immunodeficiency thus I know the HOPE monoclonal antibodies can bring to patients, hence why I would love to help build a biomanufacturing factory dedicated to producing monoclonal antibodies and mRNA vaccines.Hugoenhttps://pages.htgaa.org/2026b/michellie-hernandez/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/michellie-hernandez/week-01-hw-project-ideas/index.htmlAuthor: Dr. Michellie Hernandez, MD Assisted by Chat-GPT for content clarity and Gemini for estimated feasibility calculations. Content draft and conception was all done by Dr. Michellie Hernandez, MD Released September 20, 2026 (1) What you would like to biomanufacture and why? I would love to help build a monoclonal antibody and mRNA biomanufacturing factory that I would like to call the “HOPE” Factory.https://pages.htgaa.org/2026b/michellie-hernandez/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/michellie-hernandez/week-02-hw-dna-design/index.htmlAuthor: Dr. Michellie Hernandez, MD Assisted by Kernel AI and ChatGPT for tutorial use of Kernel and broaden understanding. Content ideation and creation was done without AI and solely done by Dr. Michellie Hernandez Release date: September 27, 2026 Part 0: Set up in Kernelhttps://pages.htgaa.org/2026b/michellie-hernandez/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/michellie-hernandez/week-03-hw-cell-free/index.htmlAuthor: Dr. Michellie Hernandez, MD Assisted by ChatGPT for tutorial and broaden understanding. Content ideation and creation was done without AI and solely done by Dr. Michellie Hernandez Release date: October 5, 2026 A. COMPARE CELL-FREE REACTION COMPOSITIONS Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Potassium Glutamate PanOx system: 200 mM PanOx SP system: 175 mM Cytomim system: 130 mM Magnesium Glutamate PanOx system: 0 PanOx SP system: 20 mM Cytomim system: 8 mMhttps://pages.htgaa.org/2026b/michellie-hernandez/week-04-lab-cloud-lab/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/michellie-hernandez/week-04-lab-cloud-lab/index.htmlAuthor: Michellie Hernandez CONTRIBUTING HYPOTHESIS – I hypothesize that mimicking the microenvironment composition where the desired protein to biomanufacture occurs naturally and adjust the components of its composition to the host lysate that is used to enhance proper folding of the desired protein within the host lysate. In the case of the coopetition, enzymatic ratios of naturally occuring microenvironment composition where the desired protein is produced and folded should be adjusted to functionally equivalent enzymes in the E. Coli. The ratios and proportions of the composition should be similar within the E. coli lysate. I hypothesize that this might enhance proper folding of the protein. Thus for cell free protein synthesis (CFPS) of reteplase the microenvironment to mimic will be the interior of mammalian Endoplasmic Reticulum, while CFPS of Petase the microenvironment to mimic will be the cytosol of Ideonella sakainsis where Petase occurs naturally. In CFSP of sfGFP the natural micro environment composition to mimic will be the cytosol of the Aequorea Victoria jellyfish.https://pages.htgaa.org/2026b/michellie-hernandez/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/michellie-hernandez/final-project/index.html