https://pages.htgaa.org/2026b/mohammad-aazim/week-02-hw-dna-design/index.htmlDNA Design & Expression Construct Part 0: Open-Source Software Setup & Workspace Note on Software: Per MIT course instructions regarding Kernel accounts, this DNA design was constructed and verified using Benchling (alongside open-source plasmid editing tools ApE / VectorBee). Repository / Project Name: PhaC1-Halomonas-HTBAA Live Benchling Construct Link: HTBAA HW2 PhaC1 Expression Construct on Benchling Construct Description: This live Benchling sequence file contains the complete, fully annotated, 1,762 bp 5’ → 3’ expression cassette for Polyhydroxyalkanoate Synthase Class I (PhaC1) designed for Halomonas bluephagenesis. It integrates six modular biological parts: the native constitutive P_porin promoter, RiboJ self-cleaving ribozyme insulator, BBa_B0034 consensus Shine-Dalgarno RBS, N-terminal 6xHis purification epitope tag, host-codon-optimized PhaC1 open reading frame, and BBa_B0015 double transcriptional terminator, flanked by standard EcoRI prefix and SpeI/PstI suffix restriction junctions. Part 1: Define Your Expression Target Building directly upon Homework #1 (biomanufacturing Polyhydroxyalkanoate [PHA] bioplastics for campus dining utensils), this construct encodes the core enzyme required for bioplastic polymerization.Hugoen