https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/index.htmlAbout me Hello! My Name is Mohammed Irfan <3 Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-01-hw-project-ideas/index.html1. What I would like to biomanufacture and why The Product I propose to biomanufacture Cardiovascular-Optimized Cultivated Beef, a cultivated meat product whose nutritional composition is intentionally engineered rather than simply reproducing that of conventional beef.https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/final-project/index.htmlhttps://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-02-hw-dna-design/index.htmlPart 0: Set up in Kernel My work is located in the HTBAA 2026 workspace, in the repository Mohammed Irfan B under the folder HW_2 Part 1: Define your expression target What molecule are we expressing? My HW1 product beef muscle tissue enriched in omega-3 fatty acids and pre-loaded with β-carotene is not itself a single molecule we can express. Instead, we are engineering the bovine muscle progenitor cells to produce the enzymes that synthesize these two components. This gives us five target proteins, organized into two functional modules:https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/mohammed-irfan-bazeer-ahamed/week-03-hw-cell-free/index.htmlA. Compare Cell-Free Reaction Compositions 1. Why do potassium glutamate and magnesium glutamate concentrations differ between compositions? Which is more sensitive? Different roles → different optimal concentrations Potassium glutamate is mainly an ionic-environment reagent. K⁺ is the dominant intracellular cation and glutamate the dominant anion in E. coli, so K-glutamate is used to recreate cytoplasmic ionic strength/osmolarity and support general enzyme and ribosome activity. Its response curve is broad: Jewett & Swartz found maximum protein production at 175 mM potassium glutamate, but the optimum sits on a fairly flat plateau, and yields were “nearly insensitive to ammonium glutamate concentrations between 0 and 100 mM.” In other words, you can be off by tens of mM and lose little activity.