https://pages.htgaa.org/2026b/mohammedhussein-baqer-naji-albaghdadi/week-02-hw-dna-design/index.htmlPart 1: Expression target Molecule: A tagged proinsulin precursor called His-R-IP (10.85 kDa; theoretical mass 10,848 Da). It is converted on a Ni-NTA column into the mature product, desB30-insulin (about 5.7 kDa). Sequence: The construct is derived from human proinsulin >(UniProt P01308), with these changes (Fig. above): • The native signal peptide is replaced by a 6×His tag followed by a GGGG flexible linker and an arginine (R13). The arginine lets trypsin remove the tag and leave the native insulin N-terminus.Hugoen