{
  "reverification_date_WIB": "2026-10-02",
  "execution": "Read-only local file comparisons; no new Kernel tool run and no new biological data",
  "baseline": {
    "length_bp": 8734,
    "GC_percent": 40.25646897183421,
    "longest_homopolymer_nt": 8,
    "DNA_sha256": "23c372fad614d39adb670cf5291f002524fd956d518e39186b48047c7d81eba1"
  },
  "native_CDS": {
    "length_bp": 786,
    "GC_percent": 37.659033078880405,
    "longest_homopolymer_nt": 6,
    "DNA_sha256": "f39e94ef48fbfd98c320cae57cdd859fac1ca239c2e995a340013afe59f24a3a"
  },
  "external_candidate_CDS": {
    "length_bp": 786,
    "GC_percent": 42.49363867684478,
    "longest_homopolymer_nt": 7,
    "DNA_sha256": "1c6160793711da702eae16da1fa266116ddf5b88cbc25268418c0bba4921159c"
  },
  "protein_length_without_terminal_stop_aa": 261,
  "checks": {
    "v2_length_8734_bp": true,
    "v2_DNA_hash_matches_previous_QC": true,
    "v2_is_circular_in_GenBank_metadata": true,
    "native_length_786_bp": true,
    "candidate_length_786_bp": true,
    "native_v2_CDS_exact_match": true,
    "v2_cassette_matches_v1": true,
    "backbone_length_7956_bp": true,
    "replacement_arithmetic_7956_minus_175_plus_953": true,
    "unchanged_backbone_left_2683_bp": true,
    "unchanged_backbone_right_5098_bp": true,
    "native_start_ATG_stop_TAA": true,
    "candidate_start_ATG_stop_TAA": true,
    "native_no_internal_stops": true,
    "candidate_no_internal_stops": true,
    "native_translation_matches_261aa_reference": true,
    "candidate_translation_matches_261aa_reference": true,
    "52_nucleotide_substitutions": true,
    "47_synonymous_codons_changed": true,
    "RBS_spacer_ATG_junction": true,
    "typed_forward_annotation_promoter": true,
    "typed_forward_annotation_ribosome-binding-site": true,
    "typed_forward_annotation_5-utr": true,
    "typed_forward_annotation_cds-yes-start-yes-stop": true,
    "typed_forward_annotation_terminator": true
  },
  "checks_passed": 25,
  "checks_total": 25,
  "nucleotide_identity_percent_native_vs_candidate": 93.38422391857506,
  "native_CDS_EcoRI_sites_1based_inclusive": [
    [
      150,
      155
    ]
  ],
  "candidate_Kernel_full_reexport_hash_verified": false,
  "screening_input_full_hash_or_independent_count_verified": false,
  "Kernel_host_specific_Bacillus_optimization_completed": false,
  "instructor_acceptance_external_alternative_confirmed": false,
  "biological_function_verified": false,
  "source_generation_QC_kept_unchanged": true,
  "historical_QC_note": "October 1 generation/QC pending fields are time-specific snapshots; use this record and report for later import, screening, schematic and search evidence."
}