{
  "sample_id": "naufal_tsabitul_azmi_2026-10-05_petase_dsbc45_v1",
  "sample_type": "experimental",
  "reagent_list": {
    "amino_acid_mix_17": 1600,
    "amp": 600,
    "base_buffer": 2000,
    "cmp": 425,
    "dsbc_ecoli": 900,
    "gmp": 425,
    "hepes_koh": 900,
    "kpo_monobasic_mix": 600,
    "lysate": 5000,
    "magnesium_glutamate": 225,
    "nicotinamide": 800,
    "nuclease_free_water": 325,
    "potassium_glutamate": 2275,
    "ribose": 1500,
    "ump": 425,
    "template_petase": 2000
  },
  "metadata": {
    "ai_model": "Codex desktop",
    "protein_target": "PETase",
    "acknowledgements": "Ginkgo PETase Ribose reference Cutinase_R2PC_054; class HW3 target-specific folding framework.",
    "comments": "HW3 initial hypothesis: decrease purified DsbC from 5.0 to 4.5 uM (1000 to 900 nL of 100-uM stock), replacing the removed 100 nL with water. Keep the other PETase Ribose components fixed. Test whether TfCut2 activity is maintained sufficiently to reduce cost per functional output. The platform estimate falls from $6.79 to $6.23 per reaction as mixed; at unchanged assay conditions the activity must remain above about 91.7% of baseline to improve cost/activity. No assay result is claimed. The DNA target is PETase/TfCut2; fixed lysate, DNA and base-buffer volumes remain 5000/2000/2000 nL."
  }
}