https://pages.htgaa.org/2026b/nitin-vashisht/week-02-hw-dna-design/index.htmlHTBAA Homework #2: DNA Design From an aptamer-biosensor concept (HW1) to a concrete expression construct Prepared by: Nitin Due: Tuesday, 29 September, 2:30 p.m. ET Part 0: Set up in Kernel I do not yet have Kernel access, so I have not created the repository/folder this part asks for, and I want to be upfront about that rather than invent one. What follows is everything Kernel would normally do for Parts 1–4 — pulling real part sequences from public databases, codon-optimizing, checking reading frame, and reasoning about restriction sites and biosecurity screening — done instead with direct database lookups (NCBI/UniProt/Addgene/FPbase) and a short Python script I wrote and ran myself for the codon-optimization and sequence-integrity checks, so that every claim below is checked rather than asserted. Once I do have Kernel access, I would create a repository and a folder named nitin-theo-biosensor (to be renamed to the lastname-project convention once I set it up) and rebuild this design inside it so the part sequences and construct map are backed by Kernel’s own records rather than my manual sourcing.Hugoen