https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-01-hw-project-ideas/index.htmlBiosensor for measuring coffee quality My proposal is an optical biosensor to qualify coffee quality by detecting caffeic acid and 5-CQA, using a hydrogel immobilization matrix. Coffee is among the most important agricultural commodities grown in tropical regions. Consumers are increasingly interested in coffee quality, so demand for high quality and specialty coffee is growing. Phenolic acids, such as caffeic acid and chlorogenic acids, directly determine coffee cup quality and play an important role in coffee flavor. [1]https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-02-hw-dna-design/index.htmlPart 1 The idea is to detect caffeic acid by expressing the Fungal Bioluminescent Pathway FBP enzymes (hispidin synthase HispS, hispidin-3-hydroxylase H3H, luciferase, and caffeylpyruvate hydrolase CPH). Together, they convert caffeic acid into fungal luciferin. [1] Figure 1. Proposed pathway of fungal luciferin biosynthesis and recycling. Caffeic acid is converted to hispidin by HispS and hydroxylated by H3H, yielding 3-hydroxyhispidin (fungal luciferin) [1]https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? The concentrations of potassium glutamate and magnesium glutamate affect the cell-free reaction environment. Their optimal concentrations depend on the overall reaction formulation and the energy substrate being used. Jewett & Swartz (2004) reoptimized magnesium because PEP has a higher affinity for Mg2+ than pyruvate. PEP-based PANOx-SP used 20 mM Mg-Glu and 175 mM K-Glu, whereas pyruvate-based Cytomim used 8 mM Mg-Glu and 130 mM K-Gluhttps://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/final-project/index.html