https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-02-hw-dna-design/index.htmlPart 1 The idea is to detect caffeic acid by expressing the Fungal Bioluminescent Pathway FBP enzymes (hispidin synthase HispS, hispidin-3-hydroxylase H3H, luciferase, and caffeylpyruvate hydrolase CPH). Together, they convert caffeic acid into fungal luciferin. [1] Figure 1. Proposed pathway of fungal luciferin biosynthesis and recycling. Caffeic acid is converted to hispidin by HispS and hydroxylated by H3H, yielding 3-hydroxyhispidin (fungal luciferin) [1]Hugoen