https://pages.htgaa.org/2026b/renata-marizza-garcia-joaquin/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? The concentrations of potassium glutamate and magnesium glutamate affect the cell-free reaction environment. Their optimal concentrations depend on the overall reaction formulation and the energy substrate being used. Jewett & Swartz (2004) reoptimized magnesium because PEP has a higher affinity for Mg2+ than pyruvate. PEP-based PANOx-SP used 20 mM Mg-Glu and 175 mM K-Glu, whereas pyruvate-based Cytomim used 8 mM Mg-Glu and 130 mM K-GluHugoen