https://pages.htgaa.org/2026b/ruben-goncalves/index.htmlAbout me No, I am not a Drosophila Melanogaster, I am a Medical Biotechnology student from Portugal. I’m fascinated by the natural world and its molecules, its complexity and ingenuity: from plants to brains and everything in between.Hugoenhttps://pages.htgaa.org/2026b/ruben-goncalves/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/week-01-hw-project-ideas/index.htmlThe cover image depicts the 3D molecular structure of the engineered plant defensin RsAFP2. (1) What you would like to biomanufacture and why? A leave-on topical hydrogel containing a re-engineered RsAFP2 plant defensin peptide to fight cutaneous fungal infections and restore skin microbiome balance.https://pages.htgaa.org/2026b/ruben-goncalves/project-follow-up/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/project-follow-up/index.htmlHere I plan to add new findings/ideas for my final project.https://pages.htgaa.org/2026b/ruben-goncalves/alternative-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/alternative-ideas/index.htmlThese are some alternative ideas I had for the final project: Relaxing Sound Chewing Gum A chewing gum that produces relaxing sounds conducted via bones. The more relaxed the chewing rythm, the more relaxing the sounds become… Be careful, don’t fall asleep or you can swallow it. Actually, not a problem (I guess) - it’s not plastic!https://pages.htgaa.org/2026b/ruben-goncalves/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/week-02-hw-dna-design/index.htmlPart 0: Set up in Kernel ruben_goncalves/goncalves-antifungal_peptide Part 1: Define your expression target What molecule are you expressing? I’m expressing an engineered plant peptide: the RsAFP2[G9R/V39R]. What is the sequence? The amino acid sequence for the wild-type defensin Rs-AFP2 from Raphanus sativus was obtained from UniProtKB (Accession: P30230, Entry: DEF1_RAPSA; https://www.uniprot.org/uniprotkb/P30230/entry):https://pages.htgaa.org/2026b/ruben-goncalves/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/week-03-hw-cell-free/index.htmlThis is a first draft with AI generated content that is still under review. A. COMPARE CELL-FREE REACTION COMPOSITIONS 1.​ Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? The optimal concentrations of potassium glutamate and magnesium glutamate differ across cell-free protein synthesis (CFPS) formulations due to several biochemical and biophysical factors:https://pages.htgaa.org/2026b/ruben-goncalves/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/ruben-goncalves/final-project/index.html