https://pages.htgaa.org/2026b/sanjey-kumar-prabath-udaya-kumar/week-01-hw-project-ideas/index.htmlProject Proposal: On-Demand, Cell-Free Recombinant Antivenom What do you want to make? I want to make cell-free on-demand antivenom, specifically for snake venom in India. India accounts for roughly 50,000 to 60,000 snakebite deaths per year (nearly half the global total), predominantly driven by four species— the Spectacled Cobra (Naja naja), Common Krait (Bungarus caeruleus), Russell’s Viper (Daboia russelii), and Saw-scaled Viper (Echis carinatus). The public health centers in remote regions where snake attacks are fairly common lack the ability to store equine-derived polyvalent antivenom in the 2 to 8 degrees celsius conditions due to lack of proper instruments or access to consistent electricity. In addition, the antivenom risks anaphylaxis in the patient, and contains mostly non-specific horse antibodies. Hence, a lyophilized recombinant nanobody cocktail. By coupling nanobodies with cell-free technology, the production shifts from massive centralized livestock facilities to on-demand synthesis at rural clinics, reconstituting freeze-dried reaction tubes with local water whenever a patient arrives.Hugoen