https://pages.htgaa.org/2026b/shakera-karolia/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/shakera-karolia/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/shakera-karolia/week-01-hw-project-ideas/index.htmlPhoto by atwena Goodman on Unsplash Homework 1: An elephant-memory-inspired neuroprotective biologic for dementia What do I want to make, and why? Elephants show exceptional long-term social and spatial memory. That’s worth investigating for people affected by Alzheimer’s disease and other dementias, which are devastating for both patients and the families who watch a loved one disappear in front of them. Roughly 55 million people currently live with dementia worldwide, a number projected to almost double to 78 million by 2030 and reach 139 million by 2050 as the world’s population ages, with the majority of cases already occurring in low- and middle-income countries (Alzheimer’s Disease International, 2015).https://pages.htgaa.org/2026b/shakera-karolia/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/shakera-karolia/week-02-hw-dna-design/index.htmlAn Elephant-Memory-Inspired Neuroprotective Biologic for Dementia Shakera Karolia | BioClub Tokyo Node | 29 September 2026 Part 0: Set up in Kernel All design work for this assignment was completed in the HTGAA 2026 workspace on Asimov Kernel under the repository SHAKERA_KAROLIA, housed within the folder Karolia-project. The assembled construct is named PKMzeta_CFPS_Expression_Cassette. The GenBank export file (PKMzeta_CFPS_Expression_Cassette.gb) accompanies this submission as a separate file.https://pages.htgaa.org/2026b/shakera-karolia/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/shakera-karolia/week-03-hw-cell-free/index.htmlHTBAA Week 3 — Designing a Cell-Free Reaction Composition Section 3 (A–C) report — for submission on the homework website (version 2, 13 October 2026) Targets: sfGFP · PETase (TfCut2) · Reteplase Objective: lowest specific cost — $ per gram (sfGFP) or $ per unit of activity (PETase, Reteplase) Reading notes. All costs use the coopetition price list. Items marked [VERIFY] are not yet confirmed and are listed in Section 11. The three designs are hypotheses with stated tests, not predicted winners. Companion files: the workbook HTBAA_HW3_CFPS_Costing.xlsx (every figure below is a live formula there) and the CFPS Design Toolkit web page (Section 8), which recomputes these designs and checks them against this report.https://pages.htgaa.org/2026b/shakera-karolia/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/shakera-karolia/final-project/index.html