https://pages.htgaa.org/2026b/sheila-ramani/index.htmlAbout me I hold an MSc in Biotechnology and am driven by a deep passion for research and continuous learning. My focus lies in synthetic biology — a field I find endlessly compelling for its ambition to understand, control, and program biological systems with precision and purpose.Hugoenhttps://pages.htgaa.org/2026b/sheila-ramani/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sheila-ramani/week-01-hw-project-ideas/index.html1)What you would like to biomanufacture and why? I would like to biomanufacturer an equivalent of olive oil in microrganisms. High in polyphenols and antioxidants (from minimal processing) — linked in multiple studies to reduced inflammation and better cardiovascular outcomes It has proven long-term epidemiological support for heart health, longevity, and reduced chronic disease risk.https://pages.htgaa.org/2026b/sheila-ramani/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sheila-ramani/week-02-hw-dna-design/index.htmlPart 0: Set up in Kernel Get oriented in the software tool before you design anything. Log in to Kernel and join the HTBAA 2026 workspace. Create a repository for your project, and inside it a folder named for you and your project, e.g. lastname-project. Spend some time exploring: open an existing part and an existing construct and notice the difference. Check out the different tools and ask Kernel AI some questions!https://pages.htgaa.org/2026b/sheila-ramani/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sheila-ramani/week-03-hw-cell-free/index.html3. ASSIGNMENT: CFPS REACTION DESIGN FOR SFGFP, PETASE, RETEPLASE A. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Potassium glutamate (~50-130+ mM): K⁺ and glutamate are the main ions in E. coli cytoplasm. At these high levels they set the ionic strength and osmotic balance that transcription and translation need.https://pages.htgaa.org/2026b/sheila-ramani/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sheila-ramani/final-project/index.html