https://pages.htgaa.org/2026b/sheila-ramani/week-03-hw-cell-free/index.html3. ASSIGNMENT: CFPS REACTION DESIGN FOR SFGFP, PETASE, RETEPLASE A. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Potassium glutamate (~50-130+ mM): K⁺ and glutamate are the main ions in E. coli cytoplasm. At these high levels they set the ionic strength and osmotic balance that transcription and translation need.Hugoen