Hey! Im Sila, a junior biotech student from Paraguay, and i love synthetic biology! My interests goes from synthetic morphogenesis to protein design, linear and non-linear circuits and plant biotechnology.
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See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!
I would like to make a system for in vivo directed evolution usign CRISPR guided mutagenesis coupled with a neuromorphic circuits for concentration positive/negative feedback. Idealy, for an enzyme that creates a substrate case, the neuromorphic circuit would be sensing the concentration of the substrate using a sensor, such that, if the concentration is high enough, it would repress/inhibit the CRISPR-guided mutagenesis part. With this part you could target some catalytic or binding domain.
The system could be applied for other cases; transcription factor optimization, by sensing the products of the targeted promoter; and binder design (with a well designed feedback system) ?
I belive this could be very useful! any lab could incorporate this without complex equipment or multiple costs for library synthesis and screening.
Any living system could potentially applied it. I think microbial platforms such as E. coli or yeasts, and mammalian cells such as CHO would be nice.
Into the system goes a CRISPR guided mutagenic system, the sgRNA, the protein to optimize, the sensor, a ribonucleoprotein and the repressor of the mutagenic system. Ideally a sensor to know when the system stopped could be interesting.
For assembly the plasmids, culturing and screening.
A well designed system that works is enough. To make it interchanble the people would need to find/design a corresponding sensor to the substrate.
It could take from 4 months to a year i think. It depends if everything works well or if no.
hi