https://pages.htgaa.org/2026b/sofya-rakitina/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/sofya-rakitina/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sofya-rakitina/week-01-hw-project-ideas/index.html(1) What you would like to biomanufacture and why? With this project, I was really lost in what to make for a while, but I was definitely hoping to produce something within the realm of materials, so I ended up having a quick chat with some people in the lab about what I could do and was told about BC and how that could be a great starting point. I’m considering designing something related to tech, or improving it, whether that’s tactile interfacing, or extensions of HCI through hardware.https://pages.htgaa.org/2026b/sofya-rakitina/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sofya-rakitina/week-02-hw-dna-design/index.htmlHere’s a link to the created folder: https://kernel.asimov.com/htgaa-2026/repositories/repository/ac213b04-6666-415f-a63e-a313df44690a/folder/8dea22af-bfa2-4e02-9b92-9adb7c61c1a8 Repository: Lifefabs HTBAA 2026; folder: rakitina-bc. Part 1: Define your expression target Okay, so to reinstate it again what I want to do is to make a swappable bacterial cellulose (BC) sensor insert for a wearable, sort of mouse-like input device, with Komagataeibacter (possibly) producing the cellulose and an engineered melanin-producing organism providing pigmentation/conductive functionality. This also made me realise that I have 2 parts to my overall manufacturing system. One should be designed for enhancing/controlling bacterial cellulose production in Komagataeibacter xylinus, and then also there’s one for melanin production in engineered E. coli. As for the final material, I’m going to co-culture them to get a melanised BC sensor.https://pages.htgaa.org/2026b/sofya-rakitina/week-03-hw-cell-free/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sofya-rakitina/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Here’s how the data describes both on the https://coopetition.htgaa.org/cfps : “Potassium is the main salt needed for ribosomes to work. Glutamate replaces chloride as the counter-ion because chloride inhibits some CFPS enzymes. Getting the right K⁺ concentration is one of the biggest tuning levers in CFPS. Reteplase Reteplase needs lower K(Glu) (~250 mM) than sfGFP (~354 mM) to reduce aggregation during synthesis”https://pages.htgaa.org/2026b/sofya-rakitina/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/sofya-rakitina/final-project/index.html