https://pages.htgaa.org/2026b/sofya-rakitina/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS (20 minutes) Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? Here’s how the data describes both on the https://coopetition.htgaa.org/cfps : “Potassium is the main salt needed for ribosomes to work. Glutamate replaces chloride as the counter-ion because chloride inhibits some CFPS enzymes. Getting the right K⁺ concentration is one of the biggest tuning levers in CFPS. Reteplase Reteplase needs lower K(Glu) (~250 mM) than sfGFP (~354 mM) to reduce aggregation during synthesis”Hugoen