https://pages.htgaa.org/2026b/sukie-wang/week-03-hw-cell-free/index.htmlA. COMPARE CELL-FREE REACTION COMPOSITIONS Why does the concentration of potassium glutamate and magnesium glutamate differ between compositions? Which is more sensitive? a. Compositions I believe that magnesium and potassium glutamate differ for different reasons. Magnesium differs because of the energy substrate where PEP, and the inorganic phosphate it generatesm, combinds and sequesters Mg². Due to such, the PEP based systems is in need of a high total magnesium to keep enough avaliable Mg²⁺ for the ribosomes. Pyruvate binds magnesium weakly and produces almost no phosphate. Because of this, the optimal avaliable magnesium level is reached with even less than half the total magnesium.Hugoen