https://pages.htgaa.org/2026b/tan-rendy/index.htmlAbout me Contact info Homework, Labs, and Projects See the nav bar on the left to look through my work for How To Biomanufacture Almost Anything (Fall 2026)!Hugoenhttps://pages.htgaa.org/2026b/tan-rendy/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/tan-rendy/week-01-hw-project-ideas/index.htmlEngineering Halomonas for Enhanced PHA Production and Its Biomedical Devices Applications (1) What do you want to make? My previous research dealt with the optimization of fermentation conditions to produce PHA using wild type Bacillus megaterium. The PHA molecules can be intended as biomaterial for biomedical devices, such as tissue engineering scaffold, surgical sutures, and wound dressing film. PHA is synthesized and accumulated by microorganisms as a form of stored carbon and energy. It is a family of polymers with varying numbers of monomers, which lead to different characteristics, namely mechanical strength, flexibility, degradation rate, molecular weight, crystallinity, surface property, etc. Essential uses of PHA for biomedical devices include biocompatibility, controlled biodegradability, and ability to be broken down into non-toxic compounds. The molecule itself is particularly interesting for me due to its versatile applications and variability based on the monomers and potential combination with other polymers.https://pages.htgaa.org/2026b/tan-rendy/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/tan-rendy/week-02-hw-dna-design/index.htmlRepository name: Tan Rendy Part 1: Define your expression target What molecule are you expressing? A protein, an RNA, or an enzyme (or small set of enzymes) for a pathway. If your HW1 product is a material, food, or chemical, identify the protein(s) that produce it and design for those. The target is poly(3-hydroxybutyrate) (PHB), a biodegradable bioplastic produced through a biosynthetic pathway. Since PHB is a polymer rather than a protein, the design will express three enzymes involved in its biosynthesis: phaA (β-ketothiolase), phaB (acetoacetyl-CoA reductase), and phaC (PHA synthase). These enzymes work together to convert acetyl-CoA into PHB. The goal is to increase PHB production in Halomonas bluephagenesis TD02.https://pages.htgaa.org/2026b/tan-rendy/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/tan-rendy/final-project/index.html