https://pages.htgaa.org/2026b/vitalia-espinoza/index.htmlBIO ATMA Living Matter · Space · Experience Designer and researcher exploring the relationship between living matter, spatial design, and immersive experiences. BIO ATMA is a research framework investigating biomaterials as a medium for imagining new forms of temporary, experimental, and sensory spatial experiences. Research Exploring how living materials can become spatial systems rather than simply objects or surfaces. Experimentation Material tests, cultivation, transformation, observation, and prototyping.Hugoenhttps://pages.htgaa.org/2026b/vitalia-espinoza/final-project/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/vitalia-espinoza/final-project/index.htmlhttps://pages.htgaa.org/2026b/vitalia-espinoza/week-01-hw-project-ideas/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/vitalia-espinoza/week-01-hw-project-ideas/index.htmlHTBAA Homework #1 Grown Artifact Biological Fabrication through Designed Growth 1. What would I like to biomanufacture and why? I propose to biomanufacture an ephemeral three-dimensional sculptural artifact combining a lightweight digitally designed scaffold with a biological membrane grown from bacterial cellulose. The proposed artifact would be approximately 1 meter tall and 50–60 cm wide. Its geometry would be vertical, volumetric, abstract, and organic, positioned somewhere between an architectural structure and a biological form.https://pages.htgaa.org/2026b/vitalia-espinoza/week-02-hw-dna-design/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/vitalia-espinoza/week-02-hw-dna-design/index.htmlPart 0 — Set up in Kernel I created my project in the HTBAA 2026 workspace using the vitalia-espinoza-bacterial-cellulose repository and the vitalia-espinoza-bcsd folder. Part 1 — Define your expression target What molecule are you expressing? I am designing the expression of BcsD, also referred to as AcsD, a component of the bacterial cellulose synthase system. I selected BcsD because it is associated with the organization and crystallization of cellulose during bacterial cellulose biosynthesis, connecting the genetic design to my goal of studying the organization of a grown cellulose-based artifact.https://pages.htgaa.org/2026b/vitalia-espinoza/week-03-hw-cell-free-reaction/index.htmlMon, 01 Jan 0001 00:00:00 +0000https://pages.htgaa.org/2026b/vitalia-espinoza/week-03-hw-cell-free-reaction/index.htmlThis homework explores the design and optimization of cell-free protein synthesis (CFPS) reactions for three target proteins: superfolder green fluorescent protein (sfGFP), PETase (TfCut2), and Reteplase. The objective is to reduce the cost per gram of functional protein, while accounting for differences in expression, folding, and activity requirements. A low-cost reaction is not necessarily the best formulation if it produces less active protein. The designs below combine literature-based reasoning with initial hypotheses developed using the CFPS Reaction Designer. The proposed modifications have not yet been experimentally validated.