https://pages.htgaa.org/2026b/vitalia-espinoza/week-03-hw-cell-free-reaction/index.htmlThis homework explores the design and optimization of cell-free protein synthesis (CFPS) reactions for three target proteins: superfolder green fluorescent protein (sfGFP), PETase (TfCut2), and Reteplase. The objective is to reduce the cost per gram of functional protein, while accounting for differences in expression, folding, and activity requirements. A low-cost reaction is not necessarily the best formulation if it produces less active protein. The designs below combine literature-based reasoning with initial hypotheses developed using the CFPS Reaction Designer. The proposed modifications have not yet been experimentally validated.Hugoen